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cell culture human multiple myeloma cell lines  (ATCC)


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    ATCC cell culture human multiple myeloma cell lines
    Cell Culture Human Multiple Myeloma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiple+myeloma+cell+lines/NCI-H929/10__2147_slash_itt__s605729-25-3-25
    Average 97 stars, based on 418 article reviews
    cell culture human multiple myeloma cell lines - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: MicroRNA-1179 targets Epiregulin (EREG) regulates the proliferation and metastasis of human multiple myeloma cells.
    Article Snippet: Multiple myeloma cell lines (U266, RPMI-8226, KMS11, JJN-3, and IM-9) were obtained from the American Type Culture Collection (ATCC, USA) and grown in RPMI-1640 media (Gibco, Ireland) with 10% foetal bovine serum (FBS, Gibco, Ireland), streptomycin (100 g/ mL) and 1% penicillin (100 U/mL) in ambient temperature at 37°C with 5% CO2.

    Article Title: Delivery of CD47-SIRPα checkpoint blocker by BCMA-directed UCAR-T cells enhances antitumor efficacy in multiple myeloma.
    Article Snippet: 16 In the treatment of relapsed or refractory multiple myeloma patients, BCMA-directed autologous 17 CAR-T cells have showed excellent anti-tumor activity.. However, their widespread application is 18 limited due to the arguably cost and time-consuming.. Multiple myeloma cells highly expressed 19 CD47 molecule and interact with the SIRPα ligand on the surface of macrophages, in which evade 20 the clearance of macrophages through the activation of “don't eat me” signal.

    Article Title: Synergistic Treatment of Prostate Cancer and Multiple Myeloma using CD46-Targeted Radioimmunotherapy Antibody–Drug Conjugates
    Article Snippet: Prostate cancer cell lines [22Rv1 (RRID: CVCL_1045), DU145 (RRID: CVCL_0105), PC3 (RRID: CVCL_0035), LNCaP (RRID: CVCL_0395), and C4-2B (RRID: CVCL_4784)] and multiple myeloma cell lines [MM.1S (RRID: CVCL_8792) and RPMI 8226 (RRID: CVCL_0014)] were obtained from ATCC.

    Article Title: RVd and CyBorD therapies remodel B-cell maturation signaling and alter immune and clonal architecture in multiple myeloma
    Article Snippet: Multiple myeloma cell lines (RPMI 8226-S, RPMI-Dox40, RPMI-MR20, and MM.1S) and additional cancer cell lines (RAJI, KG-1a, Jurkat, MOLT-4, HeLa, and NTERA-2) were obtained from ATCC (Manassas, VA).

    Concentration Assay:

    Article Title: Kinetics of Nirogacestat-Mediated Increases in B-cell Maturation Antigen on Plasma Cells Inform Therapeutic Combinations in Multiple Myeloma
    Article Snippet: .. The concentration–response relationship of nirogacestat and BCMA (mbBCMA and sBCMA) was evaluated in six multiple myeloma cell lines: MM.1R (ATCC, Cat. # CRL-2975, RRID: CVCL_8794, gift from Steven Rosen, Northwestern University), H929 (ATCC, Cat. # CRL-9068, RRID: CVCL_1600), MOLP-8 [Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ), Cat. # ACC 569, RRID: CVCL_2124], KMS-12-BM (DSMZ, Cat. # ACC 551, RRID: CVCL_1334), L-363 (DSMZ, Cat. # ACC 49, RRID: CVCL_1357), and U266 (ATCC, Cat. # TIB-196, RRID: CVCL_0566). ..

    Multiple Displacement Amplification:

    Article Title: Super enhancer acquisition drives expression of oncogenic PPP1R15B that regulates protein homeostasis in multiple myeloma
    Article Snippet: .. Multiple myeloma cell lines [NCI-H929 (ATCC #CRL-3580), U266 (ATCC #TIB-196), MM.1 S (ATCC #CRL − 2974), RPMI-8226 (ATCC #CCL-155)], Burkitt’s lymphoma cell lines [Daudi (ATCC #CCL-213), Raji (ATCC #CCL-86)], stromal cell lines [HS-5 (ATCC #CRL-3611), HS-27A (ATCC #CRL-2496)], breast cancer cell lines [MDA-MB-436 (ATCC #HTB-130), MDA-MB-468 (ATCC #HTB-132), MCF-7 (ATCC #HTB-22)], non-small cell lung cancer cell lines [NCI-H596 (ATCC #HTB-178), NCI-H520 (ATCC #HTB-182)], non-cancerous human brachial epithelial cell BEAS-2B (ATCC #CRL-3588), non-cancerous breast epithelial cell MCF-10A (ATCC #CRL-10317) and human embryonic kidney 293 T (HEK293T, ATCC #CRL-3216) were obtained from ATCC (Manassas, VA, USA). .. KMS12BM was obtained from DSMZ (#ACC 551).

    Article Title: Super enhancer acquisition drives expression of oncogenic PPP1R15B that regulates protein homeostasis in multiple myeloma.
    Article Snippet: .. Multiple myeloma cell lines [NCI-H929 (ATCC #CRL-3580), U266 (ATCC #TIB-196), MM.1 S (ATCC #CRL − 2974), RPMI-8226 (ATCC #CCL-155)], Burkitt’s lymphoma cell lines [Daudi (ATCC#CCL-213), Raji (ATCC #CCL-86)], stromal cell lines [HS-5 (ATCC #CRL-3611), HS-27A (ATCC #CRL-2496)], breast cancer cell lines [MDA-MB-436 (ATCC #HTB-130), MDA-MB-468 (ATCC #HTB-132), MCF-7 (ATCC #HTB-22)], non-small cell lung cancer cell lines [NCI-H596 (ATCC #HTB-178), NCIH520 (ATCC #HTB-182)], non-cancerous human brachial epithelial cell BEAS-2B (ATCC #CRL-3588), non-cancerous breast epithelial cell MCF10A (ATCC #CRL-10317) and human embryonic kidney 293 T (HEK293T, ATCC #CRL-3216) were obtained from ATCC (Manassas, VA, USA). .. KMS12BM was obtained from DSMZ (#ACC 551).

    Cell Culture:

    Article Title: Synergistic treatment of prostate cancer and multiple myeloma using CD46-targeted radioimmunotherapy-antibody drug conjugates
    Article Snippet: 1 Synergistic treatment of prostate cancer and multiple myeloma using CD46-targeted radioimmunotherapy-antibody drug conjugates Anil P. Bidkar,1 Scott Bidlingmaier,2 Anju Wadhwa,1 Ellis Mayne,1 Athira Raveendran,1 Shubhankar Naik,3 Cheng Xue,1 Megha Basak,1 Kondapa Naidu Bobba,1 Bonell PatiñoEscobar,4 Nancy Greenland,5 Juan Antonio Camara Serrano,6 Veronica Steri,6 Jonathan Chou,4,6 Arun Wiita,7,8,9,10 Jiang He,11,12 David M. Wilson,1,6 Rahul Aggarwal,4,6 Henry F. VanBrocklin,1,6 Youngho Seo,1,6,13 Bin Liu,2,6, # Robert R. Flavell1,6,14, # 1Department of Radiology and Biomedical Imaging, University of California, San Francisco, CA



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    Identification of constructed TCF4−/+ U266 and MMSA−1−/+ U266 stable cell lines and MMSA-1 mRNA and protein levels in TCF4−/+ U266 cells 1: U266 cells; 2: TCF4− U266-NC; 3. TCF4− U266 cells; 4: TCF4+ U266-NC; 5. TCF4+ U266 cells

    Journal: Annals of Hematology

    Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

    doi: 10.1007/s00277-026-06740-8

    Figure Lengend Snippet: Identification of constructed TCF4−/+ U266 and MMSA−1−/+ U266 stable cell lines and MMSA-1 mRNA and protein levels in TCF4−/+ U266 cells 1: U266 cells; 2: TCF4− U266-NC; 3. TCF4− U266 cells; 4: TCF4+ U266-NC; 5. TCF4+ U266 cells

    Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

    Techniques: Construct, Stable Transfection

    Dual-Luciferase Report Assay results in different U266 cells

    Journal: Annals of Hematology

    Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

    doi: 10.1007/s00277-026-06740-8

    Figure Lengend Snippet: Dual-Luciferase Report Assay results in different U266 cells

    Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

    Techniques: Luciferase

    MMSA-1 and RAS co-localization and interaction in U266 cells 1: U266 cells; 2: MMSA−1− U266-NC; 3. MMSA−1− U266 cells; 4: MMSA−1+ U266-NC; 5. MMSA−1+ U266 cells

    Journal: Annals of Hematology

    Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

    doi: 10.1007/s00277-026-06740-8

    Figure Lengend Snippet: MMSA-1 and RAS co-localization and interaction in U266 cells 1: U266 cells; 2: MMSA−1− U266-NC; 3. MMSA−1− U266 cells; 4: MMSA−1+ U266-NC; 5. MMSA−1+ U266 cells

    Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

    Techniques:

    RAS downstream related molecule expressions in different U266 cells 1: U266; 2: MMSA−1− U266-NC; 3. MMSA−1− U266; 4: MMSA−1+ U266-NC; 5. MMSA−1 + U266

    Journal: Annals of Hematology

    Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

    doi: 10.1007/s00277-026-06740-8

    Figure Lengend Snippet: RAS downstream related molecule expressions in different U266 cells 1: U266; 2: MMSA−1− U266-NC; 3. MMSA−1− U266; 4: MMSA−1+ U266-NC; 5. MMSA−1 + U266

    Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

    Techniques:

    MMSA-1’s effect on U266 cells proliferation, migration and apoptosis A showed cloning formation assay result; B showed transwell assay result; C showed apoptosis related proteins expression and D showed mitochondrial apoptosis in different U266 cells. 1: U266; 2: U266 + CCCP-1; 3. MMSA−1− U266-NC; 4. MMSA−1− U266-NC + CCCP-1; 5. MMSA−1− U266; 6: MMSA−1− U266 + CCCP-1; 7. MMSA−1+ U266-NC; 8. MMSA−1+ U266-NC + CCCP-1; 9. MMSA−1 + U266; 10. MMSA−1 + U266 + CCCP-1

    Journal: Annals of Hematology

    Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

    doi: 10.1007/s00277-026-06740-8

    Figure Lengend Snippet: MMSA-1’s effect on U266 cells proliferation, migration and apoptosis A showed cloning formation assay result; B showed transwell assay result; C showed apoptosis related proteins expression and D showed mitochondrial apoptosis in different U266 cells. 1: U266; 2: U266 + CCCP-1; 3. MMSA−1− U266-NC; 4. MMSA−1− U266-NC + CCCP-1; 5. MMSA−1− U266; 6: MMSA−1− U266 + CCCP-1; 7. MMSA−1+ U266-NC; 8. MMSA−1+ U266-NC + CCCP-1; 9. MMSA−1 + U266; 10. MMSA−1 + U266 + CCCP-1

    Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

    Techniques: Migration, Cloning, Tube Formation Assay, Transwell Assay, Expressing

    Adhesion molecules and angiogenesis factors levels in different U266 cells A showed qRT-PCR result and B showed Western Blot result of adhesion molecules; C showed angiogenesis factors levels. 1: U266 cells, 2: MMSA−1− U266-NC; 3. MMSA−1− U266; 4. MMSA−1+ U266-NC; 5. MMSA−1+ U266

    Journal: Annals of Hematology

    Article Title: MMSA-1 is regulated by Wnt/TCF4 and involved in multiple myeloma progression and invasion via RAS/RAF signaling pathway

    doi: 10.1007/s00277-026-06740-8

    Figure Lengend Snippet: Adhesion molecules and angiogenesis factors levels in different U266 cells A showed qRT-PCR result and B showed Western Blot result of adhesion molecules; C showed angiogenesis factors levels. 1: U266 cells, 2: MMSA−1− U266-NC; 3. MMSA−1− U266; 4. MMSA−1+ U266-NC; 5. MMSA−1+ U266

    Article Snippet: Human multiple myeloma cell line U266 and 293 T were purchased from Wuhan Procell Life Science&Technology Co., Ltd, both of which have been identified by Short Tandem Repeat (STR).

    Techniques: Quantitative RT-PCR, Western Blot